Supercritical Fluid Chromatography
نویسنده
چکیده
Since there is an enormous volume of information on the separation of antibiotics in the literature, readers should be able to Rnd HPLC conditions for almost any antibiotic of interest. Readers are also encouraged to consult the ofRcial compendia for analysis of bulk or formulated drugs. For analysis of biological samples, the samples may be directly injected with a column switching technique instead of employing liquid}liquid or solid-phase extraction. For sensitive detection, drugs may be subjected to preor post-column derivatization, especially with a Suorescent chromophore. Diastereomeric derivatization is useful for analysis of chiral drugs.Mass spectrometric (MS) detection is another way to increase sensitivity. Indeed, cephem and macrolide antibiotics are analysed with HPLC-MS to detect minute amount of drugs. For cephem antibiotics, capillary HPLC has been coupled with mass spectrometric detection.
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